Difference between revisions of "Part:BBa K3874003"

 
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We add BBa_K243004(GGSG), a linker between our Sericin repeat. The protein will be folded well with the linker.
 
We add BBa_K243004(GGSG), a linker between our Sericin repeat. The protein will be folded well with the linker.
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[[File:T--BJ101ID--3.png|400px|thumb|left|Figure 1. The Schematic cartoon of the DNA construct of BBa_K3874001.]]
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[[File:T--BJ101ID--4.png|400px|thumb|left|Figure 2. Western Blot Data prove IPTG 0.5mM in 18℃ and cultivating for 20h is the most proper condition and we use this condition in latter experiment..]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 06:00, 21 October 2021


To add Linker between single repeat

We add BBa_K243004(GGSG), a linker between our Sericin repeat. The protein will be folded well with the linker.

Figure 1. The Schematic cartoon of the DNA construct of BBa_K3874001.
Figure 2. Western Blot Data prove IPTG 0.5mM in 18℃ and cultivating for 20h is the most proper condition and we use this condition in latter experiment..

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]