Difference between revisions of "Part:BBa K3829012"
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| + | The composite Part is used to express PETase. SS secretes PETase out of the cell, while anchor protein anchor 4609 anchor it on the cell surface. This part is based on BBa_K3829010, replacing GFP with PETase. | ||
<p>Anchor proteins 4609 was screened by model prediction and yeGFP characterization. We then used it for the following experiment. On the basis of <a href="https://parts.igem.org/Part:BBa_K3829010">BBa_K3829010</a>, we replaced PETase with yeGFP to obtain the composite parts BBa_K3829012.</p> | <p>Anchor proteins 4609 was screened by model prediction and yeGFP characterization. We then used it for the following experiment. On the basis of <a href="https://parts.igem.org/Part:BBa_K3829010">BBa_K3829010</a>, we replaced PETase with yeGFP to obtain the composite parts BBa_K3829012.</p> | ||
Revision as of 05:47, 21 October 2021
P-ss-PETase-V5tag-Anchor protein 4609-T
The composite Part is used to express PETase. SS secretes PETase out of the cell, while anchor protein anchor 4609 anchor it on the cell surface. This part is based on BBa_K3829010, replacing GFP with PETase.
Anchor proteins 4609 was screened by model prediction and yeGFP characterization. We then used it for the following experiment. On the basis of BBa_K3829010, we replaced PETase with yeGFP to obtain the composite parts BBa_K3829012.
Sequence and Features