Difference between revisions of "Part:BBa K3739026"

 
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<partinfo>BBa_K3739026 short</partinfo>
 
<partinfo>BBa_K3739026 short</partinfo>
  
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sandwich
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This is another anchored proteins onto membranes through LamB and use his tag to comformation. We use <partinfo>BBa_K3739056</partinfo> and his tag to verify LamB's function which can anchor proteins onto membranes.
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===Biology===
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LamB is an anchor protein from Vibrio Species, which can anchor its passenger protein to the cell membrane. It has been widely used in cell-surface display. The linker is a meaningless short peptide that we use as a control. The linker is fused with LamB used as a sandwich-anchoring motif so that the linker can be displayed on the surface of VnDX.
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===Characterization===
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====1. Identification====
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After receiving the synthesized DNA, PCR was done to certify that the plasmid was correct, and the experimental results were shown in figure1.
  
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===Usage and Biology===
 
  
 
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Revision as of 05:29, 21 October 2021


LamB1-linker-LamB2-his

This is another anchored proteins onto membranes through LamB and use his tag to comformation. We use BBa_K3739056 and his tag to verify LamB's function which can anchor proteins onto membranes.

Biology

LamB is an anchor protein from Vibrio Species, which can anchor its passenger protein to the cell membrane. It has been widely used in cell-surface display. The linker is a meaningless short peptide that we use as a control. The linker is fused with LamB used as a sandwich-anchoring motif so that the linker can be displayed on the surface of VnDX.

Characterization

1. Identification

After receiving the synthesized DNA, PCR was done to certify that the plasmid was correct, and the experimental results were shown in figure1.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 612
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 760