Difference between revisions of "Part:BBa K3739019"
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<partinfo>BBa_K3739019 short</partinfo>
 
<partinfo>BBa_K3739019 short</partinfo>
  
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This is an anchored proteins onto membranes through LamB and use GFP to comformation. We use <partinfo>BBa_3739048</partinfo> and GFP to verify LamB' s function which can anchor proteins onto membranes.
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This is an anchored proteins onto membranes through LamB and use GFP to comformation. We use <partinfo>BBa_K3739048</partinfo> and GFP to verify LamB' s function which can anchor proteins onto membranes.
  
 
===Biology===
 
===Biology===

Revision as of 05:13, 21 October 2021


LamB-GFP

This is an anchored proteins onto membranes through LamB and use GFP to comformation. We use BBa_K3739048 and GFP to verify LamB' s function which can anchor proteins onto membranes.

Biology

LamB is an anchor protein from Vibrio Species, which can anchor its passenger protein to the cell membrane. It has been widely used in cell-surface display. GFP is green fluorescent protein from jellyfish Aequorea Victoria, which has been widely used as reporter for decades. GFP is fused at C terminal with LamB so that GFP may be displayed on the surface of VnDX.

Characterization

1. Identification

After receiving the synthesized DNA, PCR was done to certify that the plasmid was correct, and the experimental results were shown in figure1.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 528
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 676
    Illegal SapI.rc site found at 1246