Difference between revisions of "Part:BBa K3036005:Experience"

 
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As for the killing switch, we refer to the arabinos-induced suicide system of 2019-BNU_China, which uses arabinos-induced promoter (pBAD) to express the toxic protein MazF. So when the patient takes in arabinos, the engineering bacteria will commit a suicide.
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When we repeat the experimental methods provided by 2019-NEU_China, we found that the results are not good. We repeat 5 times and no expected outcome was achieved. As we thought maybe we failed to build the part, our advisor Xiaoting Luan reminded us that our bacteria grow very fast——2h to achieve the logarithmic phase, 4h to reached the plateau phase, 6h to recession phase.
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[[File:Growth curve EcN.png|500px|center|left|alt text]]
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We also got in touch with 2019-BNU_China and discussed the method. So we shorten the experiment time and after we did that, the outcome proved that our part was effective.
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[[File:Killing switch proof1.png|200px|thumb|left|Bacteria are in the logarithmic phase. It can be seen that the CFU of bacteria decreased significantly after arabinose induction.]]
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[[File:Killing switch proof2.png|200px|thumb|right|Bacteria are in the logarithmic phase. It can be seen that the CFU of bacteria decreased significantly after arabinose induction.]]
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[[File:Killing switch proof3.png|200px|thumb|left|Bacteria are in the logarithmic phase. It can be seen that the CFU of bacteria decreased significantly after arabinose induction.]]
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[[File:Killing switch proof4.png|200px|thumb|right|Normal bacteria entered the decay stage, but arabinose cut down the quantity of the bacteria and in turn delayed the decay stage, so there were colonies in the induction group and no colonies in the normal group.]]
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Revision as of 03:47, 21 October 2021


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