Difference between revisions of "Part:BBa K4074049:Design"

 
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===Source===
 
===Source===
  
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-
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pSB1C from the iGEM 2021 distribution kit; B. subtilis genome, Erika of the Grossman Lab, Weiss lab
  
 
===References===
 
===References===

Latest revision as of 03:38, 21 October 2021


pSB_THRC_GIBSON


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 1187
    Illegal suffix found at 3666
    Illegal EcoRI site found at 1342
    Illegal EcoRI site found at 2367
    Illegal EcoRI site found at 3448
    Illegal XbaI site found at 2340
    Illegal PstI site found at 2328
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1187
    Illegal EcoRI site found at 1342
    Illegal EcoRI site found at 2367
    Illegal EcoRI site found at 3448
    Illegal SpeI site found at 3667
    Illegal PstI site found at 2328
    Illegal PstI site found at 3681
    Illegal NotI site found at 1193
    Illegal NotI site found at 3655
    Illegal NotI site found at 3674
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1187
    Illegal EcoRI site found at 1342
    Illegal EcoRI site found at 2367
    Illegal EcoRI site found at 3448
    Illegal BamHI site found at 2346
    Illegal XhoI site found at 171
    Illegal XhoI site found at 1063
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 1187
    Illegal suffix found at 3667
    Illegal EcoRI site found at 1342
    Illegal EcoRI site found at 2367
    Illegal EcoRI site found at 3448
    Illegal XbaI site found at 2340
    Illegal PstI site found at 2328
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 1187
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1342
    Illegal EcoRI site found at 2367
    Illegal EcoRI site found at 3448
    Illegal XbaI site found at 1202
    Illegal XbaI site found at 2340
    Illegal SpeI site found at 3667
    Illegal PstI site found at 2328
    Illegal PstI site found at 3681
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

-


Source

pSB1C from the iGEM 2021 distribution kit; B. subtilis genome, Erika of the Grossman Lab, Weiss lab

References