Difference between revisions of "Part:BBa K3873999"
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We constructed the TlpA36/PtlpA36 system to guarantee that the expression of tryptone aptamer can only happen if the temperature is above 36 degree Celsius. As for the aptamer, we are inspired by 2018 Oxford who used a riboswitch for adenine so we referred to some articles and found the riboswitch of tryptophan. It consists of a tryptone aptamer and a ribosome. | We constructed the TlpA36/PtlpA36 system to guarantee that the expression of tryptone aptamer can only happen if the temperature is above 36 degree Celsius. As for the aptamer, we are inspired by 2018 Oxford who used a riboswitch for adenine so we referred to some articles and found the riboswitch of tryptophan. It consists of a tryptone aptamer and a ribosome. | ||
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Latest revision as of 03:38, 21 October 2021
An AND gate composed of a 36℃-induced promoter and Tryptophan riboswitch as a multi input modu
We constructed the TlpA36/PtlpA36 system to guarantee that the expression of tryptone aptamer can only happen if the temperature is above 36 degree Celsius. As for the aptamer, we are inspired by 2018 Oxford who used a riboswitch for adenine so we referred to some articles and found the riboswitch of tryptophan. It consists of a tryptone aptamer and a ribosome.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NotI site found at 569 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 854
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1157
- 1000COMPATIBLE WITH RFC[1000]