Difference between revisions of "Part:BBa K3873999:Experience"

Latest revision as of 03:37, 21 October 2021

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Applications of BBa_K3873999

The part follows with the sequence of oxytocin, so we detect the expression of oxytocin to prove its effectitveness.

By using ELISA to detect the extracellular oxytocin at 2h, 4h and 6h after induction with 0,0.001,0.01,0.1g/L tryptophan LB solution, we found that the expression level of oxytocin increased with time and induced concentration.

Detect the extracellular oxytocin at 2h, 4h and 6h after induction with 0,0.001,0.01,0.1g/L tryptophan LB solution and the expression level of oxytocin increased with time and induced concentration(Control=pet28a).

User Reviews

UNIQ1e3a1bf65141aae5-partinfo-00000000-QINU UNIQ1e3a1bf65141aae5-partinfo-00000001-QINU

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 ===Applications of BBa_K3873999===
-We used 0g/L, 0.001g/L and 1g/L tryptamine solution to induce the expression of oxytocin in the engineering bacteria and detected the intracellular and extracellular levels of oxytocin at 6 h respectively by Elisa.
+The part follows with the sequence of oxytocin, so we detect the expression of oxytocin to prove its effectitveness.
-The results showed that the expression level of oxytocin increased with time. In addition, the higher the induced concentration is, the more oxytocin will be expressed.
+[[File:OT-MODEL.png|800px|thumb|center]]
-Moreover, the amount of intracellular oxytocin is lower than the amount of extracellular oxytocin, indicating that our signal peptide does play a role.
+By using ELISA to detect the extracellular oxytocin at 2h, 4h and 6h after induction with 0,0.001,0.01,0.1g/L tryptophan LB solution, we found that the expression level of oxytocin increased with time and induced concentration.
-[[File:36℃-induced_promoter_and_Tryptophan_riboswitch.png|600px|thumb|center|proof1]]
+[[File:Prof2.png|500px|thumb|center|Detect the extracellular oxytocin at 2h, 4h and 6h after induction with 0,0.001,0.01,0.1g/L tryptophan LB solution and the expression level of oxytocin increased with time and induced concentration(Control=pet28a). ]]
-Based on the above experimental results, we wanted to further explore the changes in the expression level of oxytocin over time and find the most suitable concentration of tryptophan to induce the expression. By detecting the extracellular oxytocin at 2h, 4h and 6h after induction with 0,0.001,0.01,0.1g/L tryptophan LB solution, we found that the expression level of oxytocin increased with time.
-[[File:Prof2.png|600px|thumb|center|proof2]]
 ===User Reviews===