Difference between revisions of "Part:BBa K3725022:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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how you used this part and how it worked out.
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<b>Experience</b>
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After concluding that there was not statistically significant fluorescence in dual-plasmid cells with T7 Fusarium Trigger (Part BBa_K3725070) and the original Fusarium Toehold w/ GFP Reporter (Part BBa_K3725020), Lambert iGEM decided to use a redesigned toehold switch sequence, Improved Fusarium Toehold w/ GFP Reporter (Part BBa_K3725022), obtained from NUPACK. Consequently, another dual-plasmid transformation was performed using the new switch sequence and the trigger sequence, and fluorescence was measured. The fluorescence per optical density unit measurement of cells transformed with both plasmids was greater and had a statistically significant difference in comparison to the measurements of cells transformed with only the toehold part and pUC19 plasmid.
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<center>[[File:T--Lambert GA--PhytoIPTGFluoresenceData.jpeg|500px]]</center>
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<center><I>Figure 2: This graph compares the mean fluorescence per optical density values of the Fusarium oxyspora toehold switch, positive control (pUC19), and the dual plasmid. The toehold switch and positive control have very low fluorescence per optical density values, whereas the dual plasmid has a higher fluorescence per optical density value, that is significantly different from the other two groups.</I></center>
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===Applications of BBa_K3725022===
 
===Applications of BBa_K3725022===
  

Revision as of 03:29, 21 October 2021



Experience
After concluding that there was not statistically significant fluorescence in dual-plasmid cells with T7 Fusarium Trigger (Part BBa_K3725070) and the original Fusarium Toehold w/ GFP Reporter (Part BBa_K3725020), Lambert iGEM decided to use a redesigned toehold switch sequence, Improved Fusarium Toehold w/ GFP Reporter (Part BBa_K3725022), obtained from NUPACK. Consequently, another dual-plasmid transformation was performed using the new switch sequence and the trigger sequence, and fluorescence was measured. The fluorescence per optical density unit measurement of cells transformed with both plasmids was greater and had a statistically significant difference in comparison to the measurements of cells transformed with only the toehold part and pUC19 plasmid.

T--Lambert GA--PhytoIPTGFluoresenceData.jpeg


Figure 2: This graph compares the mean fluorescence per optical density values of the Fusarium oxyspora toehold switch, positive control (pUC19), and the dual plasmid. The toehold switch and positive control have very low fluorescence per optical density values, whereas the dual plasmid has a higher fluorescence per optical density value, that is significantly different from the other two groups.


Applications of BBa_K3725022

User Reviews

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