Difference between revisions of "Part:BBa J73028"

Latest revision as of 03:27, 21 October 2021

Protein Coding Sequence for Phycocyanobilin lyase subunit CpcT

Gene coding sequence for the phycocyanobilin lyase cpcT. This enzyme binds phycocyanobilin (PCB) chromophore to 'Cys-153' of the C-phycocyanin beta subunit.

Contribution part from ECUST_China

CpcE encodes phycocyanin alpha Phycocyanobilin lyase which is one part of the Phycocyanobilin lyase catalyzing alpha phycocyanin and 3Z-phycocyanobilin. Phycocyanobilin lyase is required for the chromophorylation of α-phycocyanin to form holo-α-subunit. We further improve the expression of the lyase through optimize it in the system of yeast.

Optimized Sequence(Optimized Sequence Length:663bp,GC%:45.25):

ATGTTGTCATTAGAACAATTGGCTACCCAATTGGAATCTCCAAACTCTAGAGACAGATTGTTGGCCCTTGCTGCCTTAAGAGATGTTCCACCTCCAGAAGCTGTTCCATTGATCAAGAA

GGTTTTGTGGGATGACAATTTGCAAATCCGTTCCATGGCTGTCTTTGCTTTGGGTGTTAAGCCTACCCCAGAATGTTTCGACATTTTAGTCCAATTGTTGGAAACTGAACCAGACTACG

GTATCAGAGCTGATGCTGCTGGTGCCTTGGGTTATTTGCAAGACCAAAGAGCCTTCGCTCCATTAGTCAGAGCTTTCTACGAAGACACTGATTGGTTGGTTAGATTCTCTGCCGCTGT

TTCCTTAGGTAACTTGAAAGATGTCCGTGCTGTCGAAGTCTTGAGATCTGCTTTGTCCTCTAACGAGGTTGTTATGTTGCAAGGTGCTATTGCTGCTTTGGGTGAAATTGGTGACCCAT

CCATTGTTGAAGAAATCTTGCCATTCGTCACTGCTGAAGACTGGTTGATTAGACAAAGATTAGCCGAAGCCCTAGGTAACTTGCCATCTCCAAAGACCCAATCCGCACTAGAATACTT

GGCTAAGGATGAACACCCACACGTTGCTGCCGCTGCTGAAATCTCTTTGCAACGTTTGGCTCAAAGATGA

If you also want to express the cpcE gene in the host of yeast, the sequence above has been optimized and was already proved to be function.

Sequence and Features