Difference between revisions of "Part:BBa K3725020:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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<b>Experience</b>
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how you used this part and how it worked out.
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The T7 Fusarium Trigger (Part BBa_K3725070) and the Fusarium Toehold w/ GFP Reporter (Part BBa_K3725020) are intended to be compatible with each other and be used in conjunction. To test the compatibility of the trigger sequence with the toehold sequence, a dual-plasmid transformation was performed, and fluorescence was measured in comparison with cells transformed with only the toehold part and pUC19 (positive control). All samples were divided by optical density to obtain a standardized unit. The fluorescence per optical density unit measurement of cells transformed with both the toehold and trigger sequences was not statistically greater in comparison to the measurements of cells transformed with only the toehold part and pUC19 plasmid.
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<center>[[File:T--Lambert GA--Fusarium1FluorescenceData.jpeg|500px]]</center>
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<center><I>Figure 2: This graph compares the mean fluorescence per optical density values of the Fusarium oxyspora toehold switch, positive control (pUC19), and the dual plasmid. The dual plasmid cells do not have greater measurement values compared to the other two groups.</I></center>
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===Applications of BBa_K3725020===
 
===Applications of BBa_K3725020===
  

Revision as of 03:19, 21 October 2021


Experience
The T7 Fusarium Trigger (Part BBa_K3725070) and the Fusarium Toehold w/ GFP Reporter (Part BBa_K3725020) are intended to be compatible with each other and be used in conjunction. To test the compatibility of the trigger sequence with the toehold sequence, a dual-plasmid transformation was performed, and fluorescence was measured in comparison with cells transformed with only the toehold part and pUC19 (positive control). All samples were divided by optical density to obtain a standardized unit. The fluorescence per optical density unit measurement of cells transformed with both the toehold and trigger sequences was not statistically greater in comparison to the measurements of cells transformed with only the toehold part and pUC19 plasmid.

T--Lambert GA--Fusarium1FluorescenceData.jpeg


Figure 2: This graph compares the mean fluorescence per optical density values of the Fusarium oxyspora toehold switch, positive control (pUC19), and the dual plasmid. The dual plasmid cells do not have greater measurement values compared to the other two groups.


Applications of BBa_K3725020

User Reviews

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