Difference between revisions of "Part:BBa K4074044"

 
 
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<partinfo>BBa_K4074044 short</partinfo>
 
<partinfo>BBa_K4074044 short</partinfo>
  
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<b> Contents: </b> BKD operon <br>
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<b> What is it?: </b> A basic part - plasmid backbone <br>
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<b> What does it do?: </b> It codes for bkd operon homology arms, which allows for integration into the B. subtilis genome at the bkd operon. <br>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 03:18, 21 October 2021


pSB_BKD

Contents: BKD operon
What is it?: A basic part - plasmid backbone
What does it do?: It codes for bkd operon homology arms, which allows for integration into the B. subtilis genome at the bkd operon.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2209
    Illegal XbaI site found at 2182
    Illegal PstI site found at 2170
    Illegal PstI site found at 3364
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2209
    Illegal PstI site found at 2170
    Illegal PstI site found at 3364
    Illegal NotI site found at 3357
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2209
    Illegal BamHI site found at 2188
    Illegal XhoI site found at 171
    Illegal XhoI site found at 1063
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2209
    Illegal XbaI site found at 2182
    Illegal PstI site found at 2170
    Illegal PstI site found at 3364
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2209
    Illegal XbaI site found at 2182
    Illegal PstI site found at 2170
    Illegal PstI site found at 3364
    Illegal NgoMIV site found at 3328
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.