Difference between revisions of "Part:BBa J73028"
| Line 2: | Line 2: | ||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_J73028 short</partinfo> | <partinfo>BBa_J73028 short</partinfo> | ||
| + | |||
| + | Gene coding sequence for the phycocyanobilin lyase cpcT. This enzyme binds phycocyanobilin (PCB) chromophore to 'Cys-153' of the C-phycocyanin beta subunit. | ||
CpcE encodes phycocyanin alpha Phycocyanobilin lyase which is one part of the Phycocyanobilin lyase catalyzing alpha phycocyanin and 3Z-phycocyanobilin. Phycocyanobilin lyase is required for the chromophorylation of α-phycocyanin to form holo-α-subunit. We further improve the expression of the lyase through optimize it in the system of yeast. | CpcE encodes phycocyanin alpha Phycocyanobilin lyase which is one part of the Phycocyanobilin lyase catalyzing alpha phycocyanin and 3Z-phycocyanobilin. Phycocyanobilin lyase is required for the chromophorylation of α-phycocyanin to form holo-α-subunit. We further improve the expression of the lyase through optimize it in the system of yeast. | ||
| Line 19: | Line 21: | ||
If you also want to express the cpcE gene in the host of yeast, the sequence above has been optimized and was already proved to be function. | If you also want to express the cpcE gene in the host of yeast, the sequence above has been optimized and was already proved to be function. | ||
| − | + | ||
| − | + | ||
<!-- --> | <!-- --> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 03:01, 21 October 2021
Protein Coding Sequence for Phycocyanobilin lyase subunit CpcT
Gene coding sequence for the phycocyanobilin lyase cpcT. This enzyme binds phycocyanobilin (PCB) chromophore to 'Cys-153' of the C-phycocyanin beta subunit.
CpcE encodes phycocyanin alpha Phycocyanobilin lyase which is one part of the Phycocyanobilin lyase catalyzing alpha phycocyanin and 3Z-phycocyanobilin. Phycocyanobilin lyase is required for the chromophorylation of α-phycocyanin to form holo-α-subunit. We further improve the expression of the lyase through optimize it in the system of yeast.
Optimized Sequence(Optimized Sequence Length:663bp,GC%:45.25):
ATGTTGTCATTAGAACAATTGGCTACCCAATTGGAATCTCCAAACTCTAGAGACAGATTGTTGGCCCTTGCTGCCTTAAGAGATGTTCCACCTCCAGAAGCTGTTCCATTGATCAAGAA
GGTTTTGTGGGATGACAATTTGCAAATCCGTTCCATGGCTGTCTTTGCTTTGGGTGTTAAGCCTACCCCAGAATGTTTCGACATTTTAGTCCAATTGTTGGAAACTGAACCAGACTACG
GTATCAGAGCTGATGCTGCTGGTGCCTTGGGTTATTTGCAAGACCAAAGAGCCTTCGCTCCATTAGTCAGAGCTTTCTACGAAGACACTGATTGGTTGGTTAGATTCTCTGCCGCTGT
TTCCTTAGGTAACTTGAAAGATGTCCGTGCTGTCGAAGTCTTGAGATCTGCTTTGTCCTCTAACGAGGTTGTTATGTTGCAAGGTGCTATTGCTGCTTTGGGTGAAATTGGTGACCCAT
CCATTGTTGAAGAAATCTTGCCATTCGTCACTGCTGAAGACTGGTTGATTAGACAAAGATTAGCCGAAGCCCTAGGTAACTTGCCATCTCCAAAGACCCAATCCGCACTAGAATACTT
GGCTAAGGATGAACACCCACACGTTGCTGCCGCTGCTGAAATCTCTTTGCAACGTTTGGCTCAAAGATGA
If you also want to express the cpcE gene in the host of yeast, the sequence above has been optimized and was already proved to be function.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]