Difference between revisions of "Part:BBa K3777013"
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| + | <br>When antibiotics are absent, aTF will bind to the inducible promoter（PI）and prevent RNA polymerase from initiating transcription, thus repressing the expression of reporter gene. If antibiotics are present, aTFs will bind with them and change their conformation. Therefore, they will no longer able to bind to the promoter, resulting in the expression of reporter gene. In our case, fluorescent signal increases only with the presence of antibiotics | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 02:48, 21 October 2021
TetR-T7(tetO)-3WJdB
Basic biosensor device for tetracycline detection.
Biology
The device can be used for tetracycline detection. In presence of erythromycin cells expressing this composite part will produce a fluorescent signal which can be correlated to the amount of compound present in the sample.
Usage
Characterizition
When antibiotics are absent, aTF will bind to the inducible promoter（PI）and prevent RNA polymerase from initiating transcription, thus repressing the expression of reporter gene. If antibiotics are present, aTFs will bind with them and change their conformation. Therefore, they will no longer able to bind to the promoter, resulting in the expression of reporter gene. In our case, fluorescent signal increases only with the presence of antibiotics
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1009 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]