Difference between revisions of "Part:BBa K3783001:Experience"

Revision as of 02:47, 21 October 2021

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Applications of BBa_K3783001

The promoter was cloned into a luciferase reporter plasmid and then transformed into a fraR expressing strain of E.coli<i/>. The repressor protein has been expressed under the lacZ promoter thus addition of IPTG results in increased amounts of repressor. The luminescence curve of the fusion promoter follows mostly as expected. There is a significant, almost threefold, repression of production of target proteins in the fraR+ strains. However, due to the presence of the pR, the proteins are produced at a massive scale, in the millions of light units.

OhioState Application

Figure 1. pR-pFraB Luciferase Reporter

User Reviews

UNIQcedae8596937838b-partinfo-00000001-QINU UNIQcedae8596937838b-partinfo-00000002-QINU

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 ===Applications of BBa_K3783001===
+The promoter was cloned into a luciferase reporter plasmid and then transformed into a fraR expressing strain of <i>E.coli<i/>. The repressor protein has been expressed under the lacZ promoter thus addition of IPTG results in increased amounts of repressor. The luminescence curve of the fusion promoter follows mostly as expected. There is a significant, almost threefold, repression of production of target proteins in the fraR+ strains. However, due to the presence of the pR, the proteins are produced at a massive scale, in the millions of light units.
 =OhioState Application=