Difference between revisions of "Part:BBa K3783000:Experience"
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| − | <p> The luminescence curve for pFraB works as expected. There is a significant decrease, almost half, in expression in fraR- strains compared to fraR+ strains. This shows it is effective in controlling expression of expressed proteins. | + | <p> The promoter was cloned into a luciferase reporter and then transformed into a strain of <i>E.coli/i> expressing the fraR repressor. The luminescence curve for pFraB works as expected. There is a significant decrease, almost half, in expression in fraR- strains compared to fraR+ strains. This shows it is effective in controlling expression of expressed proteins. The fraR repressor was expressed via the lacZ promoter. This is why addition of IPTG results in increased repression. |
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Revision as of 02:34, 21 October 2021
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Applications of BBa_K3783000
OhioState Application
The promoter was cloned into a luciferase reporter and then transformed into a strain of E.coli/i> expressing the fraR repressor. The luminescence curve for pFraB works as expected. There is a significant decrease, almost half, in expression in fraR- strains compared to fraR+ strains. This shows it is effective in controlling expression of expressed proteins. The fraR repressor was expressed via the lacZ promoter. This is why addition of IPTG results in increased repression.
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