Difference between revisions of "Help:Plasmid Backbone Availability 2009"
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| − | *If you decide to use an alternate location for the plasmid, keep in mind that after transforming and miniprepping you should use PCR to [http://openwetware.org/wiki/Making_construction_plasmid make the construction plasmid]. This step will produce PCR product containing the plasmid backbone with no insert and no uncut, circular plasmid, and can then be cut with the restriction enzymes for use. | + | *If you decide to use an alternate location for the plasmid, keep in mind that after [[Help:Transformation_Protocol|transforming]] and miniprepping you should use PCR to [http://openwetware.org/wiki/Making_construction_plasmid make the construction plasmid]. This step will produce PCR product containing the plasmid backbone with no insert and no uncut, circular plasmid, and can then be cut with the restriction enzymes for use. |
We will continue to update this page with more information and details, including more comprehensive quality control information and alternate locations. | We will continue to update this page with more information and details, including more comprehensive quality control information and alternate locations. | ||
Revision as of 19:34, 10 June 2009
**EDITING IN PROGRESS**
The following is a list of plasmid backbones that we have included as part of the 2009 distribution kit, most of which have P1010 and J04450. However, if you are having trouble with these, we have also included alternate sources for the plasmids.
- YES - The plasmid is available and correct
- YES[?] - The plasmid is available and correct, with the exception of one inconsistency in the QC results which should not be an issue
- NO - The plasmid is either unavailable or we have found that it carries an error
| Plasmid | J04450 | P1010 | Alt. Location | Insert (click for QC) |
|---|---|---|---|---|
| BBa_I51020 | YES | YES | NO | |
| BBa_I716101 | YES [?] | NO | NO | |
| BBa_J63009 | YES | YES [?] | Kit Plate 3, well 2M [more] | BBa_K165018 |
| BBa_J63010 | NO | NO | Kit Plate 3, well 2K [more] | BBa_K165001 |
| pSB1A10 | NO | NO | NO | |
| pSB1A3 | YES | YES | Kit Plate 2, well 10G [more] | BBa_I732205 |
| pSB4A5 | NO | YES | NO | |
| pSB6A1 | NO | NO | Kit Plate 2, well 14G [more] | BBa_I739001 |
| pSB1AC3 | YES [?] | YES [?] | Kit Plate 2, well 22M [more] | BBa_M1001 |
| pSB1AK3 | NO | NO | Kit Plate 3, well 20A [more] | BBa_K143053 |
| pSB1AK8 | YES [?] | NO | Kit Plate 2, well 13A [more] | BBa_I712006 |
| pSB1AT3 | YES | YES [?] | Kit Plate 3, well 1B [more] | BBa_K115001 |
| pSB1C3 | YES | YES | NO | |
| pSB3C5 | NO | YES | NO | |
| pSB4C5 | NO | YES | Kit Plate 3, well 5F [more] | BBa_K098010 |
| pSB1K3 | YES [?] | NO | NO | |
| pSB2K3 | NO | NO | Kit Plate 2, well 11B [more] | BBa_E0033 |
| pSB3K3 | NO | NO | Kit Plate 2, well 15L [more] | BBa_I714891 |
| pSB4K5 | YES [?] | NO | Kit Plate 2, well 17H [more] | BBa_T9003 |
| pSB1T3 | YES [?] | NO | NO | |
| pSB3T5 | YES [?] | YES [?] | Kit Plate 2, well 19A [more] | BBa_I715045 |
| pSB4T5 | NO | NO | NO |
- If you decide to use an alternate location for the plasmid, keep in mind that after transforming and miniprepping you should use PCR to [http://openwetware.org/wiki/Making_construction_plasmid make the construction plasmid]. This step will produce PCR product containing the plasmid backbone with no insert and no uncut, circular plasmid, and can then be cut with the restriction enzymes for use.
We will continue to update this page with more information and details, including more comprehensive quality control information and alternate locations.