Difference between revisions of "Part:BBa K4092012"
 
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<partinfo>BBa_K4092012 short</partinfo>
 
<partinfo>BBa_K4092012 short</partinfo>
  
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Toehold switches are RNA tools used for the detection of a sequence of interest. They're composed of four main parts: the first part is located at the beginning of the toehold and it is complementary to a given sequence of interest also called trigger; next, there is a hairpin-like sequence, which hides an RBS and start codon; subsequently, at the end of the hairpin, a linker sequence is used as a spacer before the next component; finally, a coding sequence for a reporter gene of choice is added after the toehold design. This toehold is functional at a temperature of 37°C as a negative control for the detection of Fusarium oxysporum (28S-18S ribosomal RNA intergenic spacer), since it has single nucleotide mutations which should not allow the expression of any reporter gene. It has a MFE of -26.70000076 (kcal/mol) and was designed using the software Toehold Switch Creator by the Tec-Monterrey 2021 team.
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Toehold switches are RNA tools used for the detection of a sequence of interest. They're composed of four main parts: the first part is located at the beginning of the toehold and it is complementary to a given sequence of interest also called trigger; next, there is a hairpin-like sequence, which hides an RBS and start codon; subsequently, at the end of the hairpin, a linker sequence is used as a spacer before the next component; finally, a coding sequence for a reporter gene of choice is added after the toehold design (not present in this part). This toehold is functional at a temperature of 37°C and works as a negative control for the detection of Fusarium oxysporum (28S-18S ribosomal RNA intergenic spacer), since it has a set of mutations every 8 bp on the SST1 gene complementary region it should not correctly interact with the toehold and thus not express any reporter gene. It was designed using the software Toehold Switch Creator by the Tec-Monterrey 2021 team.
  
  

Latest revision as of 01:55, 21 October 2021


Specificity control toehold for Fusarium oxysporum

Toehold switches are RNA tools used for the detection of a sequence of interest. They're composed of four main parts: the first part is located at the beginning of the toehold and it is complementary to a given sequence of interest also called trigger; next, there is a hairpin-like sequence, which hides an RBS and start codon; subsequently, at the end of the hairpin, a linker sequence is used as a spacer before the next component; finally, a coding sequence for a reporter gene of choice is added after the toehold design (not present in this part). This toehold is functional at a temperature of 37°C and works as a negative control for the detection of Fusarium oxysporum (28S-18S ribosomal RNA intergenic spacer), since it has a set of mutations every 8 bp on the SST1 gene complementary region it should not correctly interact with the toehold and thus not express any reporter gene. It was designed using the software Toehold Switch Creator by the Tec-Monterrey 2021 team.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]