Difference between revisions of "Part:BBa K3790004"

(Introduction)
(Introduction)
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[[File:T--Fudan--ccic8-transparent-logo.png|100px|right|2021 Fudan]]
 
[[File:T--Fudan--ccic8-transparent-logo.png|100px|right|2021 Fudan]]
  
Sso7d is a double-stranded binding protein that is linked to DNA polymerase A or DNA polymerase B to produce a fusion protein with higher synthetic efficiency compared to wild-type DNA polymerase.albA1, a double-stranded binding protein from the same species as Sso7d, Sulfolobus solfataricus, was suggested in previous studies to have a similar function to Sso7d in enhancing DNA polymerase activity. The Bst Pol selected for this experiment was DNA polymerase Ⅰ, and no previous studies have focused on whether double-stranded binding proteins can enhance the activity of DNA polymerase Ⅰ. However, we ventured to guess that the double-stranded binding protein could enhance the related activity of DNA polymerase Ⅰ, and performed the next validation experiments.
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albA1 is a double-stranded binding protein from the same species as Sso7d, Sulfolobus solfataricus, which is close to Sso7d in length and structure. So we speculate that it may have a similar function to enhance DNA polymerase activity as Sso7d.  
 
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Revision as of 01:23, 21 October 2021


albA1


Introduction

2021 Fudan

albA1 is a double-stranded binding protein from the same species as Sso7d, Sulfolobus solfataricus, which is close to Sso7d in length and structure. So we speculate that it may have a similar function to enhance DNA polymerase activity as Sso7d.

Usage and Biology

Experimental Results

Reference

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]