Difference between revisions of "Part:BBa K3790004"

(Introduction)
(Introduction)
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[[File:T--Fudan--ccic8-transparent-logo.png|100px|right|2021 Fudan]]
 
[[File:T--Fudan--ccic8-transparent-logo.png|100px|right|2021 Fudan]]
  
Sso7d is a double-stranded binding protein that is linked to DNA polymerase A or DNA polymerase B to produce a fusion protein with higher synthetic efficiency compared to wild-type DNA polymerase.DbpA, a double-stranded binding protein from the same species as Sso7d, Sulfolobus solfataricus, was suggested in previous studies to have a similar function to Sso7d in enhancing DNA polymerase activity.
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Sso7d is a double-stranded binding protein that is linked to DNA polymerase A or DNA polymerase B to produce a fusion protein with higher synthetic efficiency compared to wild-type DNA polymerase.albA1, a double-stranded binding protein from the same species as Sso7d, Sulfolobus solfataricus, was suggested in previous studies to have a similar function to Sso7d in enhancing DNA polymerase activity. The Bst Pol selected for this experiment was DNA polymerase Ⅰ, and no previous studies have focused on whether double-stranded binding proteins can enhance the activity of DNA polymerase Ⅰ. However, we ventured to guess that the double-stranded binding protein could enhance the related activity of DNA polymerase Ⅰ, and performed the next validation experiments.
The Bst Pol selected for this experiment was DNA polymerase Ⅰ, and no previous studies have focused on whether double-stranded binding proteins can enhance the activity of DNA polymerase Ⅰ. However, we ventured to guess that the double-stranded binding protein could enhance the related activity of DNA polymerase Ⅰ, and performed the next validation experiments
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Revision as of 01:14, 21 October 2021


albA1


Introduction

2021 Fudan

Sso7d is a double-stranded binding protein that is linked to DNA polymerase A or DNA polymerase B to produce a fusion protein with higher synthetic efficiency compared to wild-type DNA polymerase.albA1, a double-stranded binding protein from the same species as Sso7d, Sulfolobus solfataricus, was suggested in previous studies to have a similar function to Sso7d in enhancing DNA polymerase activity. The Bst Pol selected for this experiment was DNA polymerase Ⅰ, and no previous studies have focused on whether double-stranded binding proteins can enhance the activity of DNA polymerase Ⅰ. However, we ventured to guess that the double-stranded binding protein could enhance the related activity of DNA polymerase Ⅰ, and performed the next validation experiments.


Usage and Biology

Experimental Results

Reference

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]