Difference between revisions of "Part:BBa K4092110"

 
 
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<partinfo>BBa_K4092110 short</partinfo>
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Toehold switches are RNA tools used for the detection of a sequence of interest. They're composed of four main parts: the first part is located at the beginning of the toehold and it is complementary to a given sequence of interest also called trigger; next, there is a hairpin-like sequence, which hides an RBS and start codon; subsequently, at the end of the hairpin, a linker sequence is used as a spacer before the next component; finally, a coding sequence for a reporter gene of choice is added after the toehold design. This toehold is functional at a temperature of 37°C for the detection of Fusarium oxysporum (28S-18S ribosomal RNA intergenic spacer), has a MFE of -26.70000076 (kcal/mol) and was designed using the software Toehold Switch Creator by the Tec-Monterrey 2021 team.
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This cassette expression is regulated by a T7 promoter and expresses a red fluorescent protein when the toehold interacts with its corresponding trigger sequence.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4092110 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K4092110 parameters</partinfo>
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Latest revision as of 01:08, 21 October 2021


Toehold Fusarium 1 expression cassette with mRFP

Toehold switches are RNA tools used for the detection of a sequence of interest. They're composed of four main parts: the first part is located at the beginning of the toehold and it is complementary to a given sequence of interest also called trigger; next, there is a hairpin-like sequence, which hides an RBS and start codon; subsequently, at the end of the hairpin, a linker sequence is used as a spacer before the next component; finally, a coding sequence for a reporter gene of choice is added after the toehold design. This toehold is functional at a temperature of 37°C for the detection of Fusarium oxysporum (28S-18S ribosomal RNA intergenic spacer), has a MFE of -26.70000076 (kcal/mol) and was designed using the software Toehold Switch Creator by the Tec-Monterrey 2021 team.

This cassette expression is regulated by a T7 promoter and expresses a red fluorescent protein when the toehold interacts with its corresponding trigger sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 681
    Illegal AgeI site found at 793
  • 1000
    COMPATIBLE WITH RFC[1000]