Difference between revisions of "Part:BBa K4092109"

 
 
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<partinfo>BBa_K4092109 short</partinfo>
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Toehold switches are RNA tools used for the detection of a sequence of interest. They're composed of four main parts: the first part is located at the beginning of the toehold and it is complementary to a given sequence of interest also called trigger; next, there is a hairpin-like sequence, which hides an RBS and start codon; subsequently, at the end of the hairpin, a linker sequence is used as a spacer before the next component; finally, a coding sequence for a reporter gene of choice is added after the toehold design. This toehold is functional at a temperature of 37°C for the detection of Candidatus phytoplasma solani (PlsC gene).
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This cassette expression is regulated by a T7 promoter and expresses a monomeric red fluorescent protein when the toehold interacts with its corresponding trigger sequence.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4092109 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K4092109 parameters</partinfo>
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Latest revision as of 00:48, 21 October 2021


Toehold Bois Noir 2.1 (from team EPFL 2019) expression casette with mRFP

Toehold switches are RNA tools used for the detection of a sequence of interest. They're composed of four main parts: the first part is located at the beginning of the toehold and it is complementary to a given sequence of interest also called trigger; next, there is a hairpin-like sequence, which hides an RBS and start codon; subsequently, at the end of the hairpin, a linker sequence is used as a spacer before the next component; finally, a coding sequence for a reporter gene of choice is added after the toehold design. This toehold is functional at a temperature of 37°C for the detection of Candidatus phytoplasma solani (PlsC gene).

This cassette expression is regulated by a T7 promoter and expresses a monomeric red fluorescent protein when the toehold interacts with its corresponding trigger sequence.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 96
    Illegal AgeI site found at 677
    Illegal AgeI site found at 789
  • 1000
    COMPATIBLE WITH RFC[1000]