Difference between revisions of "Part:BBa K4080007"

 
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<partinfo>BBa_K4080007 short</partinfo>
 
<partinfo>BBa_K4080007 short</partinfo>
  
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(edit) One of two main systems designed to maximize the production levels of the chromoprotein to express overexpression under constitutive promoter
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(edit) This composite is using a constitutive family promoter and artificial terminator to enable expression under non T7 vectors. The feasibility of the experiment will be tested by overexpressing Chromoproteins, and by utilizing the biterminal display scaffold eCPX will they be localized on the outer membrane of the E.coli host. This cassette system is one of two that will be working to generate a CHOP system. Characterization of two expression systems (between T7 and non T7) is measured to select the best blueprint for the CHOP system.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 22:30, 20 October 2021


amilCP-eCPX fusion with universal expression

(edit) This composite is using a constitutive family promoter and artificial terminator to enable expression under non T7 vectors. The feasibility of the experiment will be tested by overexpressing Chromoproteins, and by utilizing the biterminal display scaffold eCPX will they be localized on the outer membrane of the E.coli host. This cassette system is one of two that will be working to generate a CHOP system. Characterization of two expression systems (between T7 and non T7) is measured to select the best blueprint for the CHOP system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]