Difference between revisions of "Part:BBa K3788017"
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===Role in natural environment=== | ===Role in natural environment=== | ||
| − | Induced by the SOS response (LexA dependent) upon DNA damages (UV, mitomycin…). This promoter allow the expression of the operon caa | + | <p>Induced by the SOS response (LexA dependent) upon DNA damages (UV, mitomycin…). This promoter allow the expression of the operon <i>caa-cal</i>. <i>caa</i> gene is coding for Colicin A (ColA) a toxin produced by E. coli strains, and active against other non-immune E.coli strains. E. coli have an imumnity protein coded by <i>cai</i> gene (-). <i>cal</i> gene is codding for a lysis protein, when this protein is produce ColA can be delivered in the extracellular medium after the E. coli cells lysis. </p> |
Revision as of 22:13, 20 October 2021
lexA-repressed promoter
This part is constituted from -35 and -10 boxes, with a LexA box and the beggining of the CDS of caa. The LexA box is a sequence that allows the binding of a tetramere of LexA that represses the trascription of the caa-cal operon.
This repression can be stopped when the SOS response is activated, due to DNA damage that activate RecA production that induce LexA's depolymersation.
Role in natural environment
Induced by the SOS response (LexA dependent) upon DNA damages (UV, mitomycin…). This promoter allow the expression of the operon caa-cal. caa gene is coding for Colicin A (ColA) a toxin produced by E. coli strains, and active against other non-immune E.coli strains. E. coli have an imumnity protein coded by cai gene (-). cal gene is codding for a lysis protein, when this protein is produce ColA can be delivered in the extracellular medium after the E. coli cells lysis.
experiment design
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]