Difference between revisions of "Part:BBa K4023000"

Revision as of 21:56, 20 October 2021

Modified MTIA

Modified MTIA codes for a metallothionein IA protein modified to possess specificity to arsenite in all its 7 metal binding sites. This part is optimized for expression in E.coli. When expressed, the metallothionein IA protein is intended to sequester arsenite entering E.coli. The part can therefore be used in whole cell remediation of arsenite.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4023000 short</partinfo>
+Modified MTIA codes for a metallothionein IA protein modified to possess specificity to arsenite in all its 7 metal binding sites. This part is optimized for expression in E.coli. When expressed, the metallothionein IA protein is intended to sequester arsenite entering E.coli. The part can therefore be used in whole cell remediation of arsenite.
+<!-- Add more about the biology of this part here
+===Usage and Biology===
 Metallothionein are small proteins containing a high percentage of conserved cysteine residues that bind a variety of different heavy metals. Metallothionein-IA is a metallothionein protein found in humans. It is broadly expressed in the liver, which matches its known function of detoxification of heavy metals. It is also known to protect against oxidative stress and carcinogens.
@@ Line 11: / Line 16: @@
 The MTIA gene has been optimized to express metallothionein IA proteins in E.coli for remediation of As III from the environment.
-<!-- Add more about the biology of this part here
-===Usage and Biology===
 <!-- -->