Difference between revisions of "Part:BBa K3498010:Experience"
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First, different concentrations were tested for a short period of time, resulting thath 1000 mM was the best concentration for production, afterwards, this concnetration was tested for different hours  
 
First, different concentrations were tested for a short period of time, resulting thath 1000 mM was the best concentration for production, afterwards, this concnetration was tested for different hours  
  
 +
 +
https://parts.igem.org/File:T-FCB-UANL--RSN3-I.png
  
 
Figure 1: Induction conditions for Rsn-3
 
Figure 1: Induction conditions for Rsn-3
 +
  
 
The results indicate that the best induction condition for Rsn-3 are 1000 mM and 12 hours of growing at 37°C with a production of 0.5070 mg/mL
 
The results indicate that the best induction condition for Rsn-3 are 1000 mM and 12 hours of growing at 37°C with a production of 0.5070 mg/mL
Line 55: Line 58:
 
Measurements were taken at 0, 1, 3, 5, 10, 15 and 30 minutes, after that, the measurements were taken every 30 minutes until 12 hours after the agitation
 
Measurements were taken at 0, 1, 3, 5, 10, 15 and 30 minutes, after that, the measurements were taken every 30 minutes until 12 hours after the agitation
  
 +
 +
https://parts.igem.org/File:T--FCB-UANL--PE.png
  
 
Figure 2: Water (negative control), Rsn-2, Rsn-3, Rsn 3-5, SDS (positive control), all agitated 30 second
 
Figure 2: Water (negative control), Rsn-2, Rsn-3, Rsn 3-5, SDS (positive control), all agitated 30 second
 +
  
 
The foamability is obtained by FV/SV where FV is foam volume and SV is solution volume. Ranaspumin-3 made a Foam volume of 4 mL with a 5 mL solution, hence its foamability is of 0.8 (relative number)
 
The foamability is obtained by FV/SV where FV is foam volume and SV is solution volume. Ranaspumin-3 made a Foam volume of 4 mL with a 5 mL solution, hence its foamability is of 0.8 (relative number)
  
 +
 +
https://parts.igem.org/File:T--FCB-UANL--E-GC.png
  
 
Figure 3: Foamability of Rsn-3
 
Figure 3: Foamability of Rsn-3
 +
  
 
Passing the 12 hours the behaviour of the foam was analyzed in order to see how durable it is
 
Passing the 12 hours the behaviour of the foam was analyzed in order to see how durable it is
 +
 +
https://parts.igem.org/File:T--FCB-UANL-E-GB.png
  
 
Figure 4: Foam volume over 12 hours
 
Figure 4: Foam volume over 12 hours
 +
  
 
After 12 hours the final volume of Rsn-3 was of 2.5, it means that the half life of its foam is even longer
 
After 12 hours the final volume of Rsn-3 was of 2.5, it means that the half life of its foam is even longer

Revision as of 20:06, 20 October 2021


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Please enter how you used this part and how it worked out.

Applications of BBa_K3498010

User Reviews

UNIQe3383b9ebe0c5cef-partinfo-00000000-QINU UNIQe3383b9ebe0c5cef-partinfo-00000001-QINU

•••••

IGEM FCB-UANL

PvanCC promotor + Rsn-3 RBS + Rsn-3.

This composite part is regulated by vanillic acid sensitive promoter PvanCC. This part contains an histidine tag used for purification. AlL assembled in pSB3K3 plasmid, a low number of copies plasmid. This part does not contain terminator

Introduction

Ranaspumin-3.

Ranaspumin-3, also known as Rsn-3 is recombinantly produced protein in E. coli Top 10. Its foam stability capacity is being tested for fire fighting foam production purposes

Results.

Protein expression in E. coli Top 10.

A characterization of the expression in E. coli was made. For this different concentrations of inducer (vanillic acid) and different final growing times were tested. Concentrations were determined by imageJ pixel density analyzer program

First, different concentrations were tested for a short period of time, resulting thath 1000 mM was the best concentration for production, afterwards, this concnetration was tested for different hours


https://parts.igem.org/File:T-FCB-UANL--RSN3-I.png

Figure 1: Induction conditions for Rsn-3


The results indicate that the best induction condition for Rsn-3 are 1000 mM and 12 hours of growing at 37°C with a production of 0.5070 mg/mL

Foamability

The foamability of the expressed Rsn-3 was determined by testing the sonicated cultures, manually agitating a 15 mL corning tube containing 5 mL of the sonicated protein and observing the volume of the foam produced Measurements were taken at 0, 1, 3, 5, 10, 15 and 30 minutes, after that, the measurements were taken every 30 minutes until 12 hours after the agitation


https://parts.igem.org/File:T--FCB-UANL--PE.png

Figure 2: Water (negative control), Rsn-2, Rsn-3, Rsn 3-5, SDS (positive control), all agitated 30 second


The foamability is obtained by FV/SV where FV is foam volume and SV is solution volume. Ranaspumin-3 made a Foam volume of 4 mL with a 5 mL solution, hence its foamability is of 0.8 (relative number)


https://parts.igem.org/File:T--FCB-UANL--E-GC.png

Figure 3: Foamability of Rsn-3


Passing the 12 hours the behaviour of the foam was analyzed in order to see how durable it is

https://parts.igem.org/File:T--FCB-UANL-E-GB.png

Figure 4: Foam volume over 12 hours


After 12 hours the final volume of Rsn-3 was of 2.5, it means that the half life of its foam is even longer