Difference between revisions of "Part:BBa K3971028"
Line 5: Line 5:
 
This composite part has been designed to insert the sps gene into the Neutral Site 3 of the <i>S. elongatus</i> UTEX 2973 genome.  
 
This composite part has been designed to insert the sps gene into the Neutral Site 3 of the <i>S. elongatus</i> UTEX 2973 genome.  
  
−
[[File:T--IISER-Pune-India--spscompo.png|thumb|700x900px|centre|Figure of the composite construct for characterizing cscB transport efficiencies under different promoters. NS1a and NS1b are the two homology arms to Neutral Site 1.]]
+
The sps gene is native to the Synechococcus sp, and the sps catalyzed reaction is the rate limiting step in sucrose biosynthesis in cyanobacteria [1]. It catalyzes the rate-limiting conversion of uridine diphosphate glucose (UDP-Glu) and fructose 6-phosphate (F6P) into sucrose 6-phosphate (S6P) in the sucrose synthesis pathway.
 +
 
 +
We aimed to overexpress the sps gene in order to increase the amount of sucrose produced by <i>S. elongatus </i> when exposed to salt stress.
 +
 
 +
[[File:T--IISER-Pune-India--spscompo.png|thumb|700x900px|centre|Figure of the composite construct for inserting the sps gene into the Neutral Site 3 of the <i>S. elongatus</i> UTEX 2973 genome. ]]
 +
 
 +
===References===
 +
 
 +
1: Du, Wei, et al. "Exploring the photosynthetic production capacity of sucrose by cyanobacteria." Metabolic engineering 19 (2013): 17-25.
 +
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 19:29, 20 October 2021


Cassette to insert sps gene in the Neutral Site 3 of the S. elongatus UTEX 2973 genome.

This composite part has been designed to insert the sps gene into the Neutral Site 3 of the S. elongatus UTEX 2973 genome.

The sps gene is native to the Synechococcus sp, and the sps catalyzed reaction is the rate limiting step in sucrose biosynthesis in cyanobacteria [1]. It catalyzes the rate-limiting conversion of uridine diphosphate glucose (UDP-Glu) and fructose 6-phosphate (F6P) into sucrose 6-phosphate (S6P) in the sucrose synthesis pathway.

We aimed to overexpress the sps gene in order to increase the amount of sucrose produced by S. elongatus when exposed to salt stress.

Figure of the composite construct for inserting the sps gene into the Neutral Site 3 of the S. elongatus UTEX 2973 genome.

References

1: Du, Wei, et al. "Exploring the photosynthetic production capacity of sucrose by cyanobacteria." Metabolic engineering 19 (2013): 17-25.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2877
    Illegal PstI site found at 644
    Illegal PstI site found at 2915
    Illegal PstI site found at 3636
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2877
    Illegal NheI site found at 3070
    Illegal PstI site found at 644
    Illegal PstI site found at 2915
    Illegal PstI site found at 3636
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2877
    Illegal BamHI site found at 1196
    Illegal XhoI site found at 2654
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2877
    Illegal PstI site found at 644
    Illegal PstI site found at 2915
    Illegal PstI site found at 3636
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2877
    Illegal PstI site found at 644
    Illegal PstI site found at 2915
    Illegal PstI site found at 3636
  • 1000
    COMPATIBLE WITH RFC[1000]