Difference between revisions of "Part:BBa K3971028"

Revision as of 19:25, 20 October 2021

Cassette to insert sps gene in the Neutral Site 3 of the S. elongatus UTEX 2973 genome.

This composite part has been designed to insert the sps gene into the Neutral Site 3 of the S. elongatus UTEX 2973 genome.

Figure of the composite construct for characterizing cscB transport efficiencies under different promoters. NS1a and NS1b are the two homology arms to Neutral Site 1.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 2877
Illegal PstI site found at 644
Illegal PstI site found at 2915
Illegal PstI site found at 3636
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 2877
Illegal NheI site found at 3070
Illegal PstI site found at 644
Illegal PstI site found at 2915
Illegal PstI site found at 3636
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 2877
Illegal BamHI site found at 1196
Illegal XhoI site found at 2654
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 2877
Illegal PstI site found at 644
Illegal PstI site found at 2915
Illegal PstI site found at 3636
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 2877
Illegal PstI site found at 644
Illegal PstI site found at 2915
Illegal PstI site found at 3636
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 This composite part has been designed to insert the sps gene into the Neutral Site 3 of the <i>S. elongatus</i> UTEX 2973 genome.
-[[File:T--IISER-Pune-India--spscassette.png|thumb|700x900px|centre|Figure of the composite construct for characterizing cscB transport efficiencies under different promoters. NS1a and NS1b are the two homology arms to Neutral Site 1.]]
+[[File:T--IISER-Pune-India--spscomp.png|thumb|700x900px|centre|Figure of the composite construct for characterizing cscB transport efficiencies under different promoters. NS1a and NS1b are the two homology arms to Neutral Site 1.]]
 <!-- Add more about the biology of this part here