Difference between revisions of "Part:BBa K3971008"
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<partinfo>BBa_K3971008 short</partinfo> | <partinfo>BBa_K3971008 short</partinfo> | ||
| − | This part is a portion of the Neutral Site 1 of the <i>Synechococcus elongatus</i> UTEX 2973 genome and can be used for homology repair-based modifications in the genome at Neutral Site 1. This was one of the homology arms to Neutral Site 1 in our composite part (BBa_K3971009) which was designed to insert a promoter upstream of a reporter (sYFP2) in order to measure the strength of the promoter, through a Cpf1 CRISPR plasmid. It was also a part of our composite part (BBa_K3971010) which was designed to test the sucrose export efficiencies of the cscB (sucrose permease) transporter under different promoters. | + | This part is a portion of the Neutral Site 1 of the <i>Synechococcus elongatus</i> UTEX 2973 genome and can be used for homology repair-based modifications in the genome at Neutral Site 1 using the pSL2680 plasmid, which is a Cpf1 based CRISPR plasmid [1].. This was one of the homology arms to Neutral Site 1 in our composite part (BBa_K3971009) which was designed to insert a promoter upstream of a reporter (sYFP2) in order to measure the strength of the promoter, through a Cpf1 CRISPR plasmid. It was also a part of our composite part (BBa_K3971010) which was designed to test the sucrose export efficiencies of the cscB (sucrose permease) transporter under different promoters. |
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| + | ===References=== | ||
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| + | 1: Cpf1 Is A Versatile Tool for CRISPR Genome Editing Across Diverse Species of Cyanobacteria. Ungerer J, Pakrasi HB. Sci Rep. 2016 Dec 21;6:39681. doi: 10.1038/srep39681. 10.1038/srep39681 PubMed 28000776 | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 19:15, 20 October 2021
Portion of the Neutral Site 1 of the Synechococcus elongatus UTEX 2973 genome.
This part is a portion of the Neutral Site 1 of the Synechococcus elongatus UTEX 2973 genome and can be used for homology repair-based modifications in the genome at Neutral Site 1 using the pSL2680 plasmid, which is a Cpf1 based CRISPR plasmid [1].. This was one of the homology arms to Neutral Site 1 in our composite part (BBa_K3971009) which was designed to insert a promoter upstream of a reporter (sYFP2) in order to measure the strength of the promoter, through a Cpf1 CRISPR plasmid. It was also a part of our composite part (BBa_K3971010) which was designed to test the sucrose export efficiencies of the cscB (sucrose permease) transporter under different promoters.
References
1: Cpf1 Is A Versatile Tool for CRISPR Genome Editing Across Diverse Species of Cyanobacteria. Ungerer J, Pakrasi HB. Sci Rep. 2016 Dec 21;6:39681. doi: 10.1038/srep39681. 10.1038/srep39681 PubMed 28000776
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 279
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 92
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 279
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 279
- 1000COMPATIBLE WITH RFC[1000]