Difference between revisions of "Part:BBa K3971025"
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| − | This part is a portion of the Neutral Site 3 of the <i>Synechococcus elongatus</i> UTEX 2973 genome and can be used for homology repair-based modifications in the genome at Neutral Site 3. This was one of the homology arms to Neutral Site 3 in our composite part BBa_K3971028 and the composite part BBa_K3971030 which were designed to insert sps and spp genes in the Neutral Site 3 of the <i>S. elongatus</i> genome respectively, under different promoters. | + | This part is a portion of the Neutral Site 3 of the <i>Synechococcus elongatus</i> UTEX 2973 genome and can be used for homology repair-based modifications in the genome at Neutral Site 3 using the pSL2680 plasmid, which is a Cpf1 based CRISPR plasmid [1]. This was one of the homology arms to Neutral Site 3 in our composite part BBa_K3971028 and the composite part BBa_K3971030 which were designed to insert sps and spp genes in the Neutral Site 3 of the <i>S. elongatus</i> genome respectively, under different promoters. |
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| + | ===References=== | ||
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| + | 1: Cpf1 Is A Versatile Tool for CRISPR Genome Editing Across Diverse Species of Cyanobacteria. Ungerer J, Pakrasi HB. Sci Rep. 2016 Dec 21;6:39681. doi: 10.1038/srep39681. 10.1038/srep39681 PubMed 28000776 | ||
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Latest revision as of 19:13, 20 October 2021
Portion of the Neutral Site 3 of the Synechococcus elongatus UTEX 2973 genome.
This part is a portion of the Neutral Site 3 of the Synechococcus elongatus UTEX 2973 genome and can be used for homology repair-based modifications in the genome at Neutral Site 3 using the pSL2680 plasmid, which is a Cpf1 based CRISPR plasmid [1]. This was one of the homology arms to Neutral Site 3 in our composite part BBa_K3971028 and the composite part BBa_K3971030 which were designed to insert sps and spp genes in the Neutral Site 3 of the S. elongatus genome respectively, under different promoters.
References
1: Cpf1 Is A Versatile Tool for CRISPR Genome Editing Across Diverse Species of Cyanobacteria. Ungerer J, Pakrasi HB. Sci Rep. 2016 Dec 21;6:39681. doi: 10.1038/srep39681. 10.1038/srep39681 PubMed 28000776
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 176
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 176
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 176
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 176
- 1000COMPATIBLE WITH RFC[1000]