Difference between revisions of "Part:BBa K678020"

 
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This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains tails at the 5' and 3' ends as shown on the figure below.
 
This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains tails at the 5' and 3' ends as shown on the figure below.
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<h2 class="pageContent-main__title">
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        Supplementary Experiment
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        <p>Group: CSU_CHINA 2021
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        <p>Author: Xingjun Zhao
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        <p>mCherry is a monomer red fluorescent protein. Experiments proved that when it is combined with exogenous protein on both C side and N side, the activity of fluorescent and the function of combined proteinare not effected and it can be used together
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            with multi types of fluorescent proteins. Due to the phosphorylation pathway is relatively long, we have added mCherry at the bottom as report gene to prove that our pathway works and is regulated by insulin.
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            <img width="193px" height="403" src="https://2021.igem.org/wiki/images/5/54/T--CSU_CHINA--tupian14.png"></p>
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        <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.1 Under Laser confocal microscopy, fluorescence of mCherry expression downstream of Tet-Off system</p>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 19:09, 20 October 2021


mCherry-module

This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains tails at the 5' and 3' ends as shown on the figure below.

Supplementary Experiment

Group: CSU_CHINA 2021

Author: Xingjun Zhao

mCherry is a monomer red fluorescent protein. Experiments proved that when it is combined with exogenous protein on both C side and N side, the activity of fluorescent and the function of combined proteinare not effected and it can be used together with multi types of fluorescent proteins. Due to the phosphorylation pathway is relatively long, we have added mCherry at the bottom as report gene to prove that our pathway works and is regulated by insulin.

Fig.1 Under Laser confocal microscopy, fluorescence of mCherry expression downstream of Tet-Off system

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 367
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 367
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 367
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 367
  • 1000
    COMPATIBLE WITH RFC[1000]