Difference between revisions of "Part:BBa K4006001"
| Line 7: | Line 7: | ||
We were able to clone this construct into our plasmid and select for transformed E. coli colonies. | We were able to clone this construct into our plasmid and select for transformed E. coli colonies. | ||
| + | https://2021.igem.org/wiki/images/3/36/T--ASU--arsCplates.jpg | ||
| − | + | Digestion of the miniprepped DNA with SOMETHING and SOMETHING should result in X bands of approximate sizes X and Y. | |
| − | + | https://2021.igem.org/wiki/images/9/93/T--ASU--arsCGel.jpg | |
| − | + | ||
| − | + | ||
Unfortunately, we were unable to successfully transform this construct into C. reinhardtii and test expression or effect on arsenic uptake in the algae. | Unfortunately, we were unable to successfully transform this construct into C. reinhardtii and test expression or effect on arsenic uptake in the algae. | ||
Revision as of 19:06, 20 October 2021
arsC
This part contained a protein coding sequence for arsenate reductase that has been codon optimized for use in the chloroplast of Chlamydomonas reinhardtii. Arsenate reductase catalyzes the reduction of arsenate [As(V)] to arsenite [As(III)]. Using 2 µM arsenate at a substrate, the Kcat of the protein has been recorded as 4.5 min(-1).
We were able to clone this construct into our plasmid and select for transformed E. coli colonies.
Digestion of the miniprepped DNA with SOMETHING and SOMETHING should result in X bands of approximate sizes X and Y.
Unfortunately, we were unable to successfully transform this construct into C. reinhardtii and test expression or effect on arsenic uptake in the algae.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]