Difference between revisions of "Part:BBa K3971024:Design"

 
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===Source===
 
===Source===
  
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blah
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Thw YFP and the double terminators (coding in both directions) are present in the iGEM 2021 Distribution Kit. We plan to get the BFP gene synthesized.
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The promoter can be amplified from the pCSCX plasmid, which was a gift from Claudia Vickers (Addgene plasmid # 63918 ; http://n2t.net/addgene:63918 ; RRID:Addgene_63918).
  
 
===References===
 
===References===

Revision as of 19:04, 20 October 2021


Promoter characterization cassette for native E.coli csc operon bidirectional promoter.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1699


Design Notes

blah


Source

Thw YFP and the double terminators (coding in both directions) are present in the iGEM 2021 Distribution Kit. We plan to get the BFP gene synthesized. The promoter can be amplified from the pCSCX plasmid, which was a gift from Claudia Vickers (Addgene plasmid # 63918 ; http://n2t.net/addgene:63918 ; RRID:Addgene_63918).

References