Difference between revisions of "Part:BBa K3971024"

Revision as of 18:58, 20 October 2021

Promoter characterization cassette for native E.coli csc operon bidirectional promoter.

This composite part has been designed to characterize the activity of the native E.coli csc operon bidirectional promoter. In order to allow for modularity in the promoter region of the cassette, two restriction sites Nde1 and BamH1 flank it, so that the promoter can be easily excised out and replaced with another and the cassette can be used to characterize that promoter.

A YFP and a BFP gene flank the promoter on both sides on opposite coding strands, and the relative fluorescence intensity can be used as a proxy for the strength of the promoter in both directions. Both fluorescence promoters have a double terminator (BBa_B0015).

Figure of the composite construct for characterizing the activity of the native E.coli csc operon bidirectional promoter.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1699

@@ Line 7: / Line 7: @@
 A YFP and a BFP gene flank the promoter on both sides on opposite coding strands, and the relative fluorescence intensity can be used as a proxy for the strength of the promoter in both directions. Both fluorescence promoters have a double terminator (BBa_B0015).
-[[File:T--IISER-Pune-India--bidirectionalcassette.png|thumb|700x900px|centre|Figure of the composite construct for characterizing the activity of the native <i>E.coli</i> csc operon bidirectional promoter. NS3a and NS3b are the two homology arms to Neutral Site 3.]]
+[[File:T--IISER-Pune-India--bidirectionalcassette.png|thumb|700x900px|centre|Figure of the composite construct for characterizing the activity of the native <i>E.coli</i> csc operon bidirectional promoter.]]
 <!-- Add more about the biology of this part here