Difference between revisions of "Part:BBa K4006001"
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Digestion of the miniprepped DNA with X and Y should result in X bands of approximate sizes X and Y. | Digestion of the miniprepped DNA with X and Y should result in X bands of approximate sizes X and Y. | ||
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Unfortunately, we were unable to successfully transform this construct into C. reinhardtii and test expression or effect on arsenic uptake in the algae. | Unfortunately, we were unable to successfully transform this construct into C. reinhardtii and test expression or effect on arsenic uptake in the algae. | ||
Revision as of 18:56, 20 October 2021
arsC
This part contained a protein coding sequence for arsenate reductase that has been codon optimized for use in the chloroplast of Chlamydomonas reinhardtii. Arsenate reductase catalyzes the reduction of arsenate [As(V)] to arsenite [As(III)]. Using 2 µM arsenate at a substrate, the Kcat of the protein has been recorded as 4.5 min(-1).
We were able to clone this construct into our plasmid and select for transformed E. coli colonies.
PICTURE OF PLATES.
Digestion of the miniprepped DNA with X and Y should result in X bands of approximate sizes X and Y.
<img src="
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Unfortunately, we were unable to successfully transform this construct into C. reinhardtii and test expression or effect on arsenic uptake in the algae.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]