Difference between revisions of "Part:BBa K2364042"
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<p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.1 Electrophoretic diagram of miR21T-LUC-4XmiR21T PCR product</p> | <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.1 Electrophoretic diagram of miR21T-LUC-4XmiR21T PCR product</p> | ||
Revision as of 18:56, 20 October 2021
Τ21
.
Supplementary Experiment
Group: CSU_CHINA 2021
Author: Xingjun Zhao
T21 is called miR21T in our project, it is a DNA sequence that can be the target of miRNA21. We have enhanced the method used by iGEM17_Greece in 2017 and inserted a piece of T21 in target gene 5’UTR and 4 pieces of T21s in 3’UTR, significantly improved the efficiency of miR21 targeting T21 and suppressing the expression of target gene, meet our feedback suppression requirement in our loop.
Fig.1 Electrophoretic diagram of miR21T-LUC-4XmiR21T PCR product
Fig.2 miR21 inhabited the expression of reporter LUC
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]