Difference between revisions of "Part:BBa K3712002"

 
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<partinfo>BBa_K3712002 short</partinfo>
 
<partinfo>BBa_K3712002 short</partinfo>
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USAGE AND BIOLOGY
  
 
Although, BLP-7 has a huge power against bacteria, its antibacterial and anti-inflammatory effects are still able to be further improved. Theoretically, increasing the net positive charge and hydrophobicity of antimicrobial peptides can improve its antibacterial ability. In this project, we carried out amino acid mutation of wild-type BLP-7 through modeling design, replaced specific amino acids to improve its hydrophobicity, so that antimicrobial peptides could better bind to bacterial cell membranes and improve its drilling ability. In this way, we expect to obtain a more stable mutant BLP-7 with better antibacterial and anti-inflammatory effects.
 
Although, BLP-7 has a huge power against bacteria, its antibacterial and anti-inflammatory effects are still able to be further improved. Theoretically, increasing the net positive charge and hydrophobicity of antimicrobial peptides can improve its antibacterial ability. In this project, we carried out amino acid mutation of wild-type BLP-7 through modeling design, replaced specific amino acids to improve its hydrophobicity, so that antimicrobial peptides could better bind to bacterial cell membranes and improve its drilling ability. In this way, we expect to obtain a more stable mutant BLP-7 with better antibacterial and anti-inflammatory effects.
  
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RESULTS
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Although the modeling team has given a good verification that 27 ELP has the temperature control effect we expect, in the future we will verify the temperature control aggregation ability of 54 ELP through experiments to further support the ELP aggregation temperature of the modeling group. The applicability of the control aggregation model.
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 18:33, 20 October 2021


Optimized BLP-7

USAGE AND BIOLOGY

Although, BLP-7 has a huge power against bacteria, its antibacterial and anti-inflammatory effects are still able to be further improved. Theoretically, increasing the net positive charge and hydrophobicity of antimicrobial peptides can improve its antibacterial ability. In this project, we carried out amino acid mutation of wild-type BLP-7 through modeling design, replaced specific amino acids to improve its hydrophobicity, so that antimicrobial peptides could better bind to bacterial cell membranes and improve its drilling ability. In this way, we expect to obtain a more stable mutant BLP-7 with better antibacterial and anti-inflammatory effects.

RESULTS

Although the modeling team has given a good verification that 27 ELP has the temperature control effect we expect, in the future we will verify the temperature control aggregation ability of 54 ELP through experiments to further support the ELP aggregation temperature of the modeling group. The applicability of the control aggregation model. Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 26
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 26
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 26
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 26
  • 1000
    COMPATIBLE WITH RFC[1000]