Difference between revisions of "Part:BBa K3739049"

 
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<partinfo>BBa_K3739049 short</partinfo>
 
<partinfo>BBa_K3739049 short</partinfo>
  
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Anchor GFP protein onto membranes through OmpA.
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This is an anchored proteins onto membranes through OmpA and use GFP to comformation. We use <partinfo>BBa_K3739049</partinfo> and GFP to verify OmpA' s function which can anchor proteins onto membranes.
  
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===Biology===
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<!-- Add more about the biology of this part here
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OmpA is an anchor protein from E. coli, which can anchor its passenger protein to the cell membrane. It has been widely used in cell-surface display. GFP is green fluorescent protein from jellyfish ''Aequorea Victoria'', which has been widely used as reporter for decades. GFP is fused at C terminal with OmpA so that GFP may be displayed on the surface of VnDX.
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===Usage and Biology===
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===Characterization===
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====1. Identification====
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After receiving the synthesized DNA, PCR was done to certify that the plasmid was correct, and the experimental results were shown in figure 1.
  
 
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Revision as of 18:27, 20 October 2021


J23100-B0030-OmpA-GFP-B0010

This is an anchored proteins onto membranes through OmpA and use GFP to comformation. We use BBa_K3739049 and GFP to verify OmpA' s function which can anchor proteins onto membranes.

Biology

OmpA is an anchor protein from E. coli, which can anchor its passenger protein to the cell membrane. It has been widely used in cell-surface display. GFP is green fluorescent protein from jellyfish Aequorea Victoria, which has been widely used as reporter for decades. GFP is fused at C terminal with OmpA so that GFP may be displayed on the surface of VnDX.

Characterization

1. Identification

After receiving the synthesized DNA, PCR was done to certify that the plasmid was correct, and the experimental results were shown in figure 1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 494
    Illegal SapI.rc site found at 920