Difference between revisions of "Part:BBa K3739021"

 
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<partinfo>BBa_K3739021 short</partinfo>
 
<partinfo>BBa_K3739021 short</partinfo>
  
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Anchor GFP protein onto membranes through OmpA.
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This is an anchored proteins onto membranes through OmpA and use GFP to comformation. We use <partinfo>BBa_K3739049</partinfo> and GFP to verify OmpA' s function which can anchor proteins onto membranes.
  
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===Biology===
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OmpA is an anchor protein from E. coli, which can anchor its passenger protein to the cell membrane. It has been widely used in cell-surface display. GFP is green fluorescent protein from jellyfish ''Aequorea Victoria'', which has been widely used as reporter for decades. GFP is fused at C terminal with OmpA so that GFP may be displayed on the surface of VnDX.
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===Characterization===
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====1. Identification====
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After receiving the synthesized DNA, PCR was done to certify that the plasmid was correct, and the experimental results were shown in figure 1.
  
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===Usage and Biology===
 
  
 
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Revision as of 18:25, 20 October 2021


OmpA-GFP

This is an anchored proteins onto membranes through OmpA and use GFP to comformation. We use BBa_K3739049 and GFP to verify OmpA' s function which can anchor proteins onto membranes.

Biology

OmpA is an anchor protein from E. coli, which can anchor its passenger protein to the cell membrane. It has been widely used in cell-surface display. GFP is green fluorescent protein from jellyfish Aequorea Victoria, which has been widely used as reporter for decades. GFP is fused at C terminal with OmpA so that GFP may be displayed on the surface of VnDX.

Characterization

1. Identification

After receiving the synthesized DNA, PCR was done to certify that the plasmid was correct, and the experimental results were shown in figure 1.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 430
    Illegal SapI.rc site found at 856