Difference between revisions of "Part:BBa K3971007:Design"
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===Source=== | ===Source=== | ||
| − | This sequence can be amplified from the S. elongatus UTEX 2973 genome. | + | This sequence can be amplified from the <i>S. elongatus</i> UTEX 2973 genome. |
===References=== | ===References=== | ||
Latest revision as of 18:15, 20 October 2021
Portion of the Neutral Site 1 of the Synechococcus elongatus UTEX 2973 genome.
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 353
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 353
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 353
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 353
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 143
Design Notes
The length was chosen such that it was at least 750bp and that there were no off-target effects of the gRNA within this sequence. The location of the gRNA target in Neutral Site 1 was also taken into account when choosing this sequence since there could be no overlap between the two. Since we used Kpn1 for cloning the cassette containing the composite part into the CRISPR plasmid, this sequence had to be chosen such that were no Kpn1 cut sites within it.
Source
This sequence can be amplified from the S. elongatus UTEX 2973 genome.