Difference between revisions of "Part:BBa K245114:Experience"
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<I>ihua_li NCTU_Formosa(2021)</I>
 
<I>ihua_li NCTU_Formosa(2021)</I>
 
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= Cloning Result =
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= Colony Result =
 
We successfully built DenTeeth with LL-37 .
 
We successfully built DenTeeth with LL-37 .
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[[File:T--NCTU_Formosa--seq_abc_pcr.jpg--seq_abc_pcr.jpg|500px|thumb|center|'''Figure 1. Colony PCR result of DenTeeth after cloning into E. coli BL21. (4685 b.p.)
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[[File:T--NCTU_Formosa--seq_abc_pcr.jpg--seq_abc_pcr.jpg|500px|thumb|center|'''Figure 1. Colony PCR result of DenTeeth after colony into E. coli BL21. (4685 b.p.)
 
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]]
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= Model =
 
= Model =
 
Through the modeling built with substituting parameters from published articles, the growth of E. coli and P. gingivalis under the effect of LL-37 would be inhibited significantly. However, the figure also shows that E. coli could still live with LL-37. Simply speaking, we successfully test the feasibility of DenTeeth and find that we can improve the DenTeeth with both biobrick design and efficiency optimization model.
 
Through the modeling built with substituting parameters from published articles, the growth of E. coli and P. gingivalis under the effect of LL-37 would be inhibited significantly. However, the figure also shows that E. coli could still live with LL-37. Simply speaking, we successfully test the feasibility of DenTeeth and find that we can improve the DenTeeth with both biobrick design and efficiency optimization model.

Revision as of 18:12, 20 October 2021


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Please enter how you used this part and how it worked out.

Applications of BBa_K245114

User Reviews

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•••••

ihua_li NCTU_Formosa(2021)

Colony Result

We successfully built DenTeeth with LL-37 .

Figure 1. Colony PCR result of DenTeeth after colony into E. coli BL21. (4685 b.p.)

Model

Through the modeling built with substituting parameters from published articles, the growth of E. coli and P. gingivalis under the effect of LL-37 would be inhibited significantly. However, the figure also shows that E. coli could still live with LL-37. Simply speaking, we successfully test the feasibility of DenTeeth and find that we can improve the DenTeeth with both biobrick design and efficiency optimization model.

Figure 2. The growth curve of E. coli and P.gingivalis
Figure 3. The growth curve of E. coli and P.gingivalis
;

BBa_K245114 NCTU-Formosa(2021) Not understood UNIQ5dda0e849ce8f608-partinfo-00000003-QINU