Difference between revisions of "Part:BBa K3971003:Design"

 
 
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===Design Notes===
 
===Design Notes===
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The gRNA target sequence was chosen such that it allowed at least 750 bp of the neutral site 1 to be present on either side of the target, and that it did not have any off-target sites in the rest of Synechococcus elongatus UTEX 2973 genome.
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The gRNA target sequence was chosen such that it allowed at least 750 bp of the neutral site 1 to be present on either side of the target, and that it did not have any off-target sites in the rest of <i>Synechococcus elongatus</i> UTEX 2973 genome.
  
  

Latest revision as of 18:10, 20 October 2021


gRNA target for Cpf1 based editing in the Neutral Site 1 of Synechococcus elongatus UTEX 2973.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 13
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gRNA target sequence was chosen such that it allowed at least 750 bp of the neutral site 1 to be present on either side of the target, and that it did not have any off-target sites in the rest of Synechococcus elongatus UTEX 2973 genome.


Source

This sequence was synthesized.

References