Difference between revisions of "Part:BBa K3971029:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | The gRNA target sequence was chosen such that it allowed at least 750 bp of the neutral site 3 to be present on either side of the target, and that it did not have any off-target sites in the rest of Synechococcus elongatus UTEX 2973 genome. | |
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===Source=== | ===Source=== | ||
| − | + | This sequence was synthesized. | |
===References=== | ===References=== | ||
Revision as of 18:08, 20 October 2021
gRNA target for Cpf1 based editing in the Neutral Site 3 of Synechococcus elongatus UTEX 2973.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The gRNA target sequence was chosen such that it allowed at least 750 bp of the neutral site 3 to be present on either side of the target, and that it did not have any off-target sites in the rest of Synechococcus elongatus UTEX 2973 genome.
Source
This sequence was synthesized.