Difference between revisions of "Part:BBa K245114:Experience"
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We successfully built DenTeeth with LL-37 .
 
We successfully built DenTeeth with LL-37 .
 
[[File:T--NCTU_Formosa--seq_abc_pcr.jpg--seq_abc_pcr.jpg|500px|thumb|center|'''Figure 1. Colony PCR result of DenTeeth after cloning into E. coli BL21. (4685 b.p.)
 
[[File:T--NCTU_Formosa--seq_abc_pcr.jpg--seq_abc_pcr.jpg|500px|thumb|center|'''Figure 1. Colony PCR result of DenTeeth after cloning into E. coli BL21. (4685 b.p.)
 +
]]
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= Cloning Result =
 +
Through the modeling built with substituting parameters from published articles, the growth of E. coli and P. gingivalis under the effect of LL-37 would be inhibited significantly. However, the figure also shows that E. coli could still live with LL-37. Simply speaking, we successfully test the feasibility of DenTeeth and find that we can improve the DenTeeth with both biobrick design and efficiency optimization model.
 +
[[File:T--NCTU_Formosa--seq_abc_pcr.jpg--seq_abc_pcr.jpg|500px|thumb|center|'''Figure 3. The growth curve of E. coli and P.gingivalis
 
]]
 
]]
 
|};
 
|};

Revision as of 18:03, 20 October 2021


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Please enter how you used this part and how it worked out.

Applications of BBa_K245114

User Reviews

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ihua_li NCTU_Formosa(2021)

Cloning Result

We successfully built DenTeeth with LL-37 .

Figure 1. Colony PCR result of DenTeeth after cloning into E. coli BL21. (4685 b.p.)

Cloning Result

Through the modeling built with substituting parameters from published articles, the growth of E. coli and P. gingivalis under the effect of LL-37 would be inhibited significantly. However, the figure also shows that E. coli could still live with LL-37. Simply speaking, we successfully test the feasibility of DenTeeth and find that we can improve the DenTeeth with both biobrick design and efficiency optimization model.

Figure 3. The growth curve of E. coli and P.gingivalis
;

BBa_K245114 NCTU-Formosa(2021) Not understood UNIQ68665eda7981600f-partinfo-00000003-QINU