Difference between revisions of "Part:BBa K3971002"

Latest revision as of 17:59, 20 October 2021

cscA - Sucrose-6-phosphate hydrolase (Invertase) from E.coli W.

Usage and Biology

cscA allows E.coli to grow on sucrose as a sole carbon source. Knockout strains of cscA could not grow on sucrose suggesting that it is essential for sucrose metabolism. Intracellular sucrose is hydrolyzed to glucose and fructose and its expression is derepressed at low sucrose concentrations (0.2 %). Overexpression of cscA alone has been shown to confer sucrose utilizing ability in different E.coli strains, however with low growth rates. [1] Overexpression has also been shown to result in leakage of the invertase, which was identified in the extracellular medium [2]. However, in wild-type strains, invertase activities are exclusively intracellular [1].

csc operon in E.coli W for sucrose metabolism. Figure adapted from: Bruschi, Michele, et al. "A transferable sucrose utilization approach for non-sucrose-utilizing Escherichia coli strains." Biotechnology advances 30.5 (2012): 1001-1010.

Team iGEM IISER Pune

References

1: Sabri, Suriana, Lars K. Nielsen, and Claudia E. Vickers. "Molecular control of sucrose utilization in Escherichia coli W, an efficient sucrose-utilizing strain." Applied and environmental microbiology 79.2 (2013): 478-487.

2: Lee, Jeong Wook, et al. "Development of sucrose-utilizing Escherichia coli K-12 strain by cloning β-fructofuranosidases and its application for L-threonine production." Applied microbiology and biotechnology 88.4 (2010): 905-913.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 255
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 ===Usage and Biology===
-cscA allows E.coli to grow on sucrose as a sole carbon source. Knockout strains of cscA could not grow on sucrose suggesting that it is essential for sucrose metabolism. Intracellular sucrose is hydrolyzed to glucose and fructose and it its expression is derepressed at low sucrose concentrations (0.2 %). Overexpression of cscA alone has been shown to confer sucrose utilizing ability in different E.coli strains, however with low growth rates. [1]
+cscA allows E.coli to grow on sucrose as a sole carbon source. Knockout strains of cscA could not grow on sucrose suggesting that it is essential for sucrose metabolism. Intracellular sucrose is hydrolyzed to glucose and fructose and its expression is derepressed at low sucrose concentrations (0.2 %). Overexpression of cscA alone has been shown to confer sucrose utilizing ability in different E.coli strains, however with low growth rates. [1]
 Overexpression has also been shown to result in leakage of the invertase, which was identified in the extracellular medium [2]. However, in wild-type strains, invertase activities are exclusively intracellular [1].