Difference between revisions of "Part:BBa K3714000"
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<partinfo>BBa_K3714000 parameters</partinfo>
 
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<b>Reference</b>
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<b>Reference</b><br/>
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1 Townshend, B., Xiang, J. S., Manzanarez, G., Hayden, E. J. & Smolke, C. D. A multiplexed, automated evolution pipeline enables scalable discovery and characterization of biosensors. Nature Communications 12, 1437, doi:10.1038/s41467-021-21716-0 (2021).
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[1] Townshend, B., Xiang, J. S., Manzanarez, G., Hayden, E. J. & Smolke, C. D. A multiplexed, automated evolution pipeline enables scalable discovery and characterization of biosensors. Nature Communications 12, 1437, doi:10.1038/s41467-021-21716-0 (2021).

Revision as of 17:46, 20 October 2021


Gardiquimod biosensor 337

The template of a newly found self-cleaving aptazyme[1], which can keep itself intact with gardiquimod present, and catalyze the self-cleavage reaction in the absence of gardiquimod. This part could be used as a switch for gene expression controlled by gardiquimod.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


K3714000.png
Figure.1 | Verification of biosensor gard-337. An in vitro transcription system was employed to test if this biosensor can response gardiquimod signal. The product was run on a denatured PAGE.

Reference
[1] Townshend, B., Xiang, J. S., Manzanarez, G., Hayden, E. J. & Smolke, C. D. A multiplexed, automated evolution pipeline enables scalable discovery and characterization of biosensors. Nature Communications 12, 1437, doi:10.1038/s41467-021-21716-0 (2021).