Difference between revisions of "Part:BBa K3739084"

 
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<partinfo>BBa_K3739084 short</partinfo>
 
<partinfo>BBa_K3739084 short</partinfo>
  
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Histidine ammonia-lyase with a his-tag is involved in catalyzing the reaction of changing L-histidine into trans-urocanate. Aly01 is a signal peptile. Direct fluorescent labeling of tetracysteine-motif-tagged secretory proteins with the biarsenical compound FlAsH is possible in situ without the need to recover the cell-free supernatant. K525998 is used for expression.
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Histidine ammonia-lyase with a his-tag is involved in catalyzing the reaction of changing L-histidine into trans-urocanate. Aly01 is a signal peptile. The proteins labeled of tetracysteine motif tag (FlAsH tag, FT in short) can be detected with the biarsenical compound FlAsH.  
  
 
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Revision as of 17:19, 20 October 2021


K525998(T7-RBS)-Aly01-his-hutH-FT

Histidine ammonia-lyase with a his-tag is involved in catalyzing the reaction of changing L-histidine into trans-urocanate. Aly01 is a signal peptile. The proteins labeled of tetracysteine motif tag (FlAsH tag, FT in short) can be detected with the biarsenical compound FlAsH.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 275
    Illegal NgoMIV site found at 711
    Illegal NgoMIV site found at 1446
    Illegal NgoMIV site found at 1682
    Illegal AgeI site found at 111
    Illegal AgeI site found at 348
    Illegal AgeI site found at 1175
    Illegal AgeI site found at 1671
  • 1000
    COMPATIBLE WITH RFC[1000]