Difference between revisions of "Part:BBa K3740043"
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<h4>2.Characterization</h4> | <h4>2.Characterization</h4> | ||
<p>As shown in Figure 3, J23100-B0034-bphS-pET RBS-bphO-rrnB T1-J23119-B0034-fcsR-rrnB T1-pSEVA331-<i>G. hansenii</i> ATCC 53582 was not significantly difference in BC film production under NIR light and dark conditions.</p> | <p>As shown in Figure 3, J23100-B0034-bphS-pET RBS-bphO-rrnB T1-J23119-B0034-fcsR-rrnB T1-pSEVA331-<i>G. hansenii</i> ATCC 53582 was not significantly difference in BC film production under NIR light and dark conditions.</p> | ||
− | [[File:szpt29.png|550px|thumb|center|Figure 3, BC film production by different strains. (a) J23100-B0034-bphS-pET RBS-bphO- | + | [[File:szpt29.png|550px|thumb|center|Figure 3, BC film production by different strains. (a) J23100-B0034-bphS-pET RBS-bphO-rrnB T1-J23119-B0034-fcsR-rrnB T1-pSEVA331-<i>G. hansenii</i> ATCC 53582; (b) pSEVA331-<i>G. hansenii</i> ATCC 53582.]] |
<h3>References</h3> | <h3>References</h3> | ||
<p>[1] Min-Hyung, Gomelsky, Mark. Near-infrared Light Responsive Synthetic c-di-GMP Module for Optogenetic Applications</p> | <p>[1] Min-Hyung, Gomelsky, Mark. Near-infrared Light Responsive Synthetic c-di-GMP Module for Optogenetic Applications</p> |
Latest revision as of 17:17, 20 October 2021
J23100-B0034-bphS-pET RBS-bphO-rrnB T1-J23119-B0034-fcsR-rrnB T1
Description
This is a device that is used to regulate the level of c-di-GMP.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 2818
Illegal NheI site found at 2841
Illegal NotI site found at 189 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 294
Illegal BglII site found at 1357
Illegal XhoI site found at 632
Illegal XhoI site found at 2489
Illegal XhoI site found at 3577 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 447
Illegal NgoMIV site found at 621
Illegal NgoMIV site found at 938
Illegal NgoMIV site found at 999
Illegal NgoMIV site found at 2678
Illegal NgoMIV site found at 2935
Illegal NgoMIV site found at 3680
Illegal AgeI site found at 2634 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 982
Illegal BsaI site found at 3574
Illegal BsaI.rc site found at 482
Illegal BsaI.rc site found at 1456
Illegal BsaI.rc site found at 3322
Illegal SapI.rc site found at 3687
2021 SZPT-China
Biology
This is a device that is used to regulate the level of c-di-GMP.
Usage
We connected J23100-B0034-bphS-pET RBS-bphO-rrnB T1 (BBa_K3740047) and J23119-B0034-fcsR-rrnB T1 (BBa_K3740052) to the expression vector pSEVA331 through the expression vector pSEVA331. Then the ligated mixture is introduced into E. coli DH5α for replication, and then the plasmid is transferred into G. hansenii ATCC 53582, so that G. hansenii ATCC 53582 can control BC production through light regulation.
Characterization
1.Identification
As shown in Figure 2, composite part BBa_K3740043 was identified successfully by PCR amplification.
2.Characterization
As shown in Figure 3, J23100-B0034-bphS-pET RBS-bphO-rrnB T1-J23119-B0034-fcsR-rrnB T1-pSEVA331-G. hansenii ATCC 53582 was not significantly difference in BC film production under NIR light and dark conditions.
References
[1] Min-Hyung, Gomelsky, Mark. Near-infrared Light Responsive Synthetic c-di-GMP Module for Optogenetic Applications