Latest revision as of 17:15, 20 October 2021

J23100-B0034-bphS-pET RBS-bphO-rrnB T1-J23110-B0034-fcsR-rrnB T1

Description

This is a device that is used to regulate the level of c-di-GMP.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 2818
Illegal NheI site found at 2841
Illegal NotI site found at 189
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 294
Illegal BglII site found at 1357
Illegal XhoI site found at 632
Illegal XhoI site found at 2489
Illegal XhoI site found at 3577
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 447
Illegal NgoMIV site found at 621
Illegal NgoMIV site found at 938
Illegal NgoMIV site found at 999
Illegal NgoMIV site found at 2678
Illegal NgoMIV site found at 2935
Illegal NgoMIV site found at 3680
Illegal AgeI site found at 2634
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 982
Illegal BsaI site found at 3574
Illegal BsaI.rc site found at 482
Illegal BsaI.rc site found at 1456
Illegal BsaI.rc site found at 3322
Illegal SapI.rc site found at 3687

2021 SZPT-China

Biology

This is a device that is used to regulate the level of c-di-GMP.

Usage

We connected J23100-B0034-bphS-pET RBS-bphO-rrnB T1 (BBa_K3740047) and J23110-B0034-fcsR-rrnB T1 (BBa_K3740049) to the expression vector pSEVA331 through the expression vector pSEVA331. Then the ligated mixture was introduced into E. coli DH5α for replication, and then the plasmid was transferred into G. hansenii ATCC 53582, so that G. hansenii ATCC 53582 could control BC production through light regulation.

Figure 1. Gene circuit of J23100-B0034-bphS-pET RBS-bphO-J23110-B0034-fcsR-rrnB T1

Characterization

1.Identification

As shown in Figure 2, composite part BBa_K3740031 was identified successfully by PCR.

Figure 2. Agarose gel image of J23100-B0034-bphS-pET RBS-bphO-rrnB T1-J23110-B0034-fcsR-rrnB T1. BBa_K3740031, 3765bp

2.Characterization

A significant difference in BC film production under NIR light and dark conditions was observed for strains, J23100-B0034-bphS-pET RBS-bphO-rrnB T1-J23110-B0034-fcsR-rrnB T1-pSEVA331-G. hansenii ATCC 53582, indicating that G. hansenii ATCC 53582 could control BC production through light regulation.

Figure 3. BC film production by different strains. (a) J23100-B0034-bphS-pET RBS-bphO-rrnB T1 -J23110-B0034-fcsR-rrnB T1-pSEVA331-G. hansenii ATCC 53582; (b)pSEVA331-G. hansenii ATCC 53582.

References

[1] Min-Hyung, Gomelsky, Mark. Near-infrared Light Responsive Synthetic c-di-GMP Module for Optogenetic Applications

@@ Line 27: / Line 27: @@
 <h4>2.Characterization</h4>
 <p>A significant difference in BC film production under NIR light and dark conditions was observed for strains, J23100-B0034-bphS-pET RBS-bphO-rrnB T1-J23110-B0034-fcsR-rrnB T1-pSEVA331-<i>G. hansenii</i> ATCC 53582, <b>indicating that <i>G. hansenii</i> ATCC 53582 could control BC production through light regulation.</b></p>
-[[File:szpt26.png|550px|thumb|center|Figure 3. BC film production by different strains. (a) J23100-B0034-bphS-pET RBS-bphO-rrB T1 -J23110-B0034-fcsR-rrB T1-pSEVA331-<i>G. hansenii</i> ATCC 53582; (b)pSEVA331-<i>G. hansenii</i> ATCC 53582.]]
+[[File:szpt26.png|550px|thumb|center|Figure 3. BC film production by different strains. (a) J23100-B0034-bphS-pET RBS-bphO-rrnB T1 -J23110-B0034-fcsR-rrnB T1-pSEVA331-<i>G. hansenii</i> ATCC 53582; (b)pSEVA331-<i>G. hansenii</i> ATCC 53582.]]
 <h3>References</h3>
 <p>[1] Min-Hyung, Gomelsky, Mark. Near-infrared Light Responsive Synthetic c-di-GMP Module for Optogenetic Applications</p>

Difference between revisions of "Part:BBa K3740031"