Difference between revisions of "Part:BBa K143075"

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AmyE 3' Integration sequence(<bbpart>BBa_K143002</bbpart>) coupled to GFP proein coding region and the registry double terminator (part <bbpart>BBa_I13401</bbpart>).
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AmyE 3' Integration sequence(<bbpart>BBa_K143002</bbpart>) coupled to GFP protein coding region and the registry double terminator (part <bbpart>BBa_I13401</bbpart>).
  
  

Latest revision as of 15:59, 20 May 2009

B. subtilis AmyE locus GFP output promoter and RBS tester


AmyE 3' Integration sequence(BBa_K143002) coupled to GFP protein coding region and the registry double terminator (part BBa_I13401).


The amyE 3' integration sequence allows integration into the B. subtilis genome at the amyE locus if the 5' amyE integration sequence(BBa_K143001) is cloned onto the 5' end of the construct.


The GFP coding region gives a fluorescent output when a promoter and RBS are placed immediately upstream of the coding region while the terminator prevents readthrough.


Sequence and Features



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644